HPLC

High-Performance Liquid Chromatography (HPLC or high-pressure liquid chromatography) is an adaptation of column chromatography. HPLC is performed at a higher flow rate, and therefore higher pressure, than classical column chromatography. This enables the use of smaller stationary phase beads with a greater surface area to volume ratio, which greatly increases the interaction of the stationary phase and components in the mobile phase.

Ultra-High-Performance Liquid Chromatography (UHPLC) is a technique used to identify, quantify and separate components of a mixture by using high pressure to push solvents through the column. In UHPLC, particle sizes less than 2 µm can be used, providing better separation than HPLC where particle size is limited to 5 µm. These smaller particles require higher pump pressures making this technique very efficient with fast analysis and higher resolution.